GBALA Posted April 16, 2013 Posted April 16, 2013 I like to check one of my fraction which was eluted with 100% hexane from column and the content is high and showing single spot on normal phase TLC. The spot lies on the solvent front as the mobile phase solvent chosen is 100% hexane. So it could be mixture of hydrocarbons. I would like to know how many compounds this mixture does contain. If reverse phase TLC would be an option what is the best mobile phase solvent system to achieve good resolution? Help would be much appreciated.
Knumbnuts Posted April 17, 2013 Posted April 17, 2013 You may be better putting this mixture through a GC.
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