F3nt0n Posted April 27, 2013 Posted April 27, 2013 Hi guis, recently I ran for the first time a Western Blot + immunodetection analysis on few specific proteins and their antibodies;Beta-actine: 1°Mc Ms β-actin / 2°GAM-HRPPCNA: 1°Mc Ms PCNA / 2°GAM-HRPOccludine: 1°Pc Rb OCC / 2°GAR-biotin / 3°s-HRPI got the following results:http://s23.postimg.org/i0jea1oyz/Occludin.jpghttp://s13.postimg.org/qg0r2ew2v/PCNA.jpghttp://s15.postimg.org/flsazmwij/PCNA_b_actine.jpghttp://s24.postimg.org/af5t0z0dx/Occludin_b_actine.jpg Since i'm quite new to this, I really wondered what those other lines were (beside the molecular ladder). The saturated lines are the proteins (of that I'm sure), but lighter ones are unknown to me.Also I wondered wether it is possible to find a copy of the right molecular ladder somewhere online, since I lost my copy.Thank you in advance
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now