Jump to content

Recommended Posts

Posted

Hi guis, recently I ran for the first time a Western Blot + immunodetection analysis on few specific proteins and their antibodies;

Beta-actine: 1°Mc Ms β-actin / 2°GAM-HRP
PCNA: 1°Mc Ms PCNA / 2°GAM-HRP
Occludine: 1°Pc Rb OCC / 2°GAR-biotin / 3°s-HRP

I got the following results:
http://s23.postimg.org/i0jea1oyz/Occludin.jpg
http://s13.postimg.org/qg0r2ew2v/PCNA.jpg
http://s15.postimg.org/flsazmwij/PCNA_b_actine.jpg
http://s24.postimg.org/af5t0z0dx/Occludin_b_actine.jpg

Since i'm quite new to this, I really wondered what those other lines were (beside the molecular ladder). The saturated lines are the proteins (of that I'm sure), but lighter ones are unknown to me.
Also I wondered wether it is possible to find a copy of the right molecular ladder somewhere online, since I lost my copy.

Thank you in advance

 

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.