kem Posted April 29, 2013 Share Posted April 29, 2013 I have recently conducted a biology experiment that requires me to calculate percent conjugation. The value that I obtained is very low. Why is bacterial conjugation so rare? Link to comment Share on other sites More sharing options...
Esrevinu Posted April 30, 2013 Share Posted April 30, 2013 (edited) It is believed that the F plasmid carries the genes responsible for conjugation/formation of the pilus, however it won't conjugate with bacteria that already have the F plasmid. One must be a donor, and one must be a receiver. The donor has the F plasmid, while the receiver does not. If a large portion of your bacteria already have the F-plasmid, there will not be a large percent of conjugation. More information about what you did (experimental set up, strains used, plasmids, etc) would be useful. Were you looking for Hfr? Regular F+? Etc... Edited April 30, 2013 by Esrevinu 1 Link to comment Share on other sites More sharing options...
CharonY Posted April 30, 2013 Share Posted April 30, 2013 Rare relative to what? Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now