ElsaW Posted May 27, 2013 Share Posted May 27, 2013 Hi everybody Has anybody ever used FAM (donor) and HEX (acceptor) as the two probes in a hybridization probe assay? Are they spectrally far enough separated from each other so the emission of FAM won't interfere with that of HEX? And also, has anybody ever used this on a Bio-Rad CFX96 instrument? Just want to make sure the instrument will be able to perform my assay. Thanx! Link to comment Share on other sites More sharing options...
gfritz Posted September 14, 2013 Share Posted September 14, 2013 Good question. FRET probes can be used.on a CFX. FAM / HEX is fine. www.youtube.com/watch?v=4JBFpXVXhyw Link to comment Share on other sites More sharing options...
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