iamleo Posted May 30, 2013 Share Posted May 30, 2013 i am trying to clone my gene in pTZ. when ever i do transformation in DH5 alpha strain i get nothing on transformed plates. am doing transformation by T/A cloning method. my ligation is ok. my cells are fine. but still cant get results. can any one guide me? Link to comment Share on other sites More sharing options...
gabevillegas Posted September 26, 2013 Share Posted September 26, 2013 can you give details on your transformation protcol? Link to comment Share on other sites More sharing options...
sona_p Posted February 13, 2014 Share Posted February 13, 2014 Try with more DNA Link to comment Share on other sites More sharing options...
chadn737 Posted February 13, 2014 Share Posted February 13, 2014 How do you know your ligation is fine? Have you tested a control plasmid? Link to comment Share on other sites More sharing options...
CharonY Posted February 13, 2014 Share Posted February 13, 2014 Sona, I would suggest that you check the dates to threads you are replying to. OP has not been active for almost an year. Link to comment Share on other sites More sharing options...
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