transformation problem

[iamleo]

i am trying to clone my gene in pTZ. when ever i do transformation in DH5 alpha strain i get nothing on transformed plates. am doing transformation by T/A cloning method. my ligation is ok. my cells are fine. but still cant get results. can any one guide me?

[gabevillegas]

can you give details on your transformation protcol?

[sona_p]

Try with more DNA

[chadn737]

How do you know your ligation is fine? Have you tested a control plasmid?

[CharonY]

Sona, I would suggest that you check the dates to threads you are replying to. OP has not been active for almost an year.