Asha Posted July 9, 2013 Posted July 9, 2013 Would 8 M urea brake up E. coli cells? If you had them in a pellet, used enough 8 M urea and only resuspended it?
CharonY Posted July 9, 2013 Posted July 9, 2013 Urea is only a chaotrope and as such does little to degrade cell wall and membrane. Without further treatment the lysis rate would be very low and not suitable for most molecular biological applications.
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