biochem. just need some pointers!

[HELPMExad]

how to calculate the amount of LDH activity as a percentage of that present before heat inactivation (i.e. to give %activity restored)?

 

incubation time (mins)

BSA

Hsp70

-ATP

+ATP

-ATP

+ATP

0

0.28

0.28

0.3

0.38

10

0.3

0.32

0.68

1.65

20

0.29

0.31

0.93

2.63

30

0.37

0.41

1.17

4.58

40

0.45

0.48

1.17

6.52

50

0.57

0.6

1.55

8.03

60

0.79

0.81

1.74

8.93

 

BSA = bovine serum albumin
HSP70 = heat shock protein (just a protein thats part of the HSP family).

At 0 the heat shock was administered and at 60 mins they've recovered, 0 minutes upwards the recovery was on

certainly didnt realise that'll happen...!