How do coat antibody in micro titer plate?

[kanagasundar87]

HI Guys,

I need ours help, How Do I coat a antigen specific antibody on the surface of the Micro titer plate ?

[CharonY]

For simple immobilization e.g. for ELISA? In most cases adsorption is sufficient on materials such as polystyrene. There are ways to improve it by surface activation or incorporation of linkers. Depends on the purpose.

[TAJohnson]

What antibody are you using? I have bought pre-coated plates from a company called Assaypro before. It really saved me time and they had an extensive selection