Conservation of culture medium

[mandri01]

Hi to everyone!

 

I am a biomedical engineer but now i'm working with cell cultures, in particular c2c12 cells.

 

My question is: how long the culture medium, and the differentiation medium, are good after I prepared it?

Now I conserve them in a fridge at 4° C.

 

Thanks

[CharonY]

Depends on composition a bit. But as a rule of thumb sterile media can be stored about 4-6 weeks for complete mixes (i.e.including serum and other supplements). The basic medium tends to be good for a bit longer. Commercial media usually have an expiry date. For self-made ones without any sensitive supplements a few months are likely to work, but then with cell cultures I would rather make fresh media rather than risk messing up cultures.