anaw Posted November 6, 2013 Share Posted November 6, 2013 Hi! my supervisor wants me to use exosomes in our model and I am trying to follow th methodof Nat. Protocols El-Andaloussi et al 2012 and Alvarez-Erviti Nat Biotech 2011 but I am not getting any silencing in the brain and I have just seen these comments in you tube and in this blog talking about fraud and saying that this papers should be retracted! who should I trust? does anybody know other protocol for brain delivery of siRNAs using exosomes? I am quite desperate! any advice would be greatly appreciated! http://metamodern.com/2011/03/22/across-the-blood-brain-barrier-with-exosomes/ Cheers Link to comment Share on other sites More sharing options...
Quant Posted March 10, 2014 Share Posted March 10, 2014 (edited) I would stop trying, there is already an ongoing investigation for scientific misconduct. Edited March 10, 2014 by Quant Link to comment Share on other sites More sharing options...
Quant Posted March 12, 2014 Share Posted March 12, 2014 (edited) Edited March 12, 2014 by Quant Link to comment Share on other sites More sharing options...
tkadm30 Posted January 21, 2017 Share Posted January 21, 2017 CRISPR is rapidly becoming a standard research tool in siRNA delivery. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813155/ Link to comment Share on other sites More sharing options...
CharonY Posted January 21, 2017 Share Posted January 21, 2017 That doesn't make sense. CRISPR is not a RNA delivey tool (and hence does not apply to OP at all). The linked paper actually explains the differences. Link to comment Share on other sites More sharing options...
tkadm30 Posted January 24, 2017 Share Posted January 24, 2017 That doesn't make sense. CRISPR is not a RNA delivey tool (and hence does not apply to OP at all). The linked paper actually explains the differences. CRISPR/Cas9 is a RNA-guided endonuclease system. The advantage of using CRISPR over RNAi (exosomes) is that CRISPR can "turn off genes at the DNA level, creating a knockout." http://scopeblog.stanford.edu/2016/05/10/crispr-or-rnai-which-gene-switch-is-better/ Link to comment Share on other sites More sharing options...
CharonY Posted January 24, 2017 Share Posted January 24, 2017 You are repeating words but do you know what they mean? What, for example is an exosome and what is the relationship specifically with RNAi? Have you checked the difference between silencing and knockout? And do you then understand why these are two unrelated mechanisms? Link to comment Share on other sites More sharing options...
tkadm30 Posted January 24, 2017 Share Posted January 24, 2017 You are repeating words but do you know what they mean? What, for example is an exosome and what is the relationship specifically with RNAi? Have you checked the difference between silencing and knockout? And do you then understand why these are two unrelated mechanisms? I understand that RNAi and CRISPR are systems for genome editing. Gene knockout occurs at the DNA level, while gene silencing is produced from enzymatic reactions. Link to comment Share on other sites More sharing options...
CharonY Posted January 24, 2017 Share Posted January 24, 2017 Genome editing implies changes on the genome level. One technique does it, the other does not. Link to comment Share on other sites More sharing options...
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