tane Posted November 27, 2013 Posted November 27, 2013 Hi,I observed dark shadows in the loading well region of the gel and extra bands in all lanes, even for blank. My usual bands still get separated as per normal. I tried loading the same prepared solutions the next day and run using the same power pack but a freshly prepared running buffer, everything goes back to normal. Please see file attached for the atypical gel image. Anyone knows what went wrong on the first day? Thanks! SDS-PAGE gel.doc
tane Posted November 28, 2013 Author Posted November 28, 2013 If the running buffer for Day 1 was contaminated, will it result in this phenomenon?
Quromen Posted December 22, 2013 Posted December 22, 2013 (edited) Contamination Edited December 22, 2013 by Quromen
Quromen Posted December 22, 2013 Posted December 22, 2013 i would tell there was contamination with nuclease that caused the smearing
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