Deferoxamine mesylate concentration

[stellaparallax]

Hi there,

Was hoping someone could offer some assistance. I need to measure deferoxamine mesylate concentration in solution. Is there any way that this can be produced spectrometrically?

[John Cuthber]

What else is present in the solution?

Is it in clean water or urine or blood serum or something?

I strongly suspect that, in the presence of excess iron, you can measure the stuff spectrophotometrically.

[CharonY]

If memory serves it is a siderophore? In that case you could consider a simple CAS (chrome azurol S) assay. Provided as John mentioned that you do not have too much in terms of interfering substances.

[stellaparallax]

Hi thanks so much for your replies. it's just in PBS.So If I added FeSO4, I'd be able to measure it fine? Does it matter if I add a large excess?

[sialic acid]

I haven't looked into any of the details, but a quick google search shows that people have done HPLC analysis on the compound. HPLC would be a fantastic and pretty reliable way to do it. Interfering proteins or not. The free form absorbs at 226 nm, so it may be a little bit of a challenge with regards to your mobile phase to make sure something wouldn't absorb there too and to get a good signal since it is a low wavelength and noise may be an issue. I have however done simple UV absorbance of carbohydrates that have poor UV absorbance and had to use 210 nm. It still worked. If you chelate it with Fe(3+), the iron bound form can absorb at 430 nm which may make it easier if you have to use an organic solvent system.

Edited December 8, 2013 by sialic acid