Plasmid Isolation

[Quromen]

Lets say there is a bacteria X with 10 different types of plasmid in it. And i want to isolate each of the plasmid and identify them. How can i isolate each of these 10 plasmid without contamination? Can it be done based on size, Centrifugation based on the kb of the plasmid? I need to know the way to isolate each plasmid separately.

Edited December 22, 2013 by Quromen

[CharonY]

Typical methods include re-isolation from gel, chromatography and equilibrium density centrifugation. Standard centrifugation would not allow separation.

[sona_p]

Perform plasmid DNA isolation that will give you all plasmids together. Then use very less amount (ngs) to transform E. coli. Grow them on selective media if they have any markers. there is a 80% chance you will be able to separately grow them and isolate them from E. coli