Capturing multiple antibodies in a single assay for immunogenicity testing

[immunologist1]

We're working on immunogenicity in my lab and we're trying to put together a simple assay that will screen for all of the antibodies at once to a PEGylated protein to screen for PEG and protein antibodies. We're injecting mice with a protein that is PEGylated and seeing if they develop antibodies.

 

We plan on coating the plates with the PEGylated protein then adding mouse serum that was incubated with the same PEGylated protein that was labeled. We hope that the antibodies can function as bridge and we will have a positive screening for antibodies regarldess if they are for PEG or the protein.

 

Just want to know if this assay will work or some possible complications that may be involved with this assay.