Identifying amount of product formed from given substances and yeast

[rasen58]

A yeast extract contains all the enzymes required for alcohol production. The extract is incubated under anaerobic conditions in 1 liter of media containing: 200 mM glucose, 20 mM ADP, 40 mM ATP, 2 mM NADH, 2mM NAD+, and 20 mM Pi (inorganic phosphates).

What is the max amount of ethanol that can be produced in these conditions?

 

I have no idea what to do at all for this problem. Is there an equation for ethanol production?

[CharonY]

Yes there is. You will have to look up the metabolic steps involved coming from glucose and ending with ethanol (there are variations, though, check the one for Saccharomyces). Then look at what you got and figure out what the limiting concentration is. That will determine the maximum yield.

[rasen58]

I found

2 ATP + glucose + 4 ADP + 2 P_i + 2 NAD⁺ 2 ADP + 2 pyruvate + 4 ATP + 2 NADH

 

Then I tried plugging in the numbers from the problem, but I'm not sure what to do after that

[CharonY]

That is the part for glycolysis. Now you need to find the reactions for ethanol fermentation.