Radioligand Binding Analysis

[Peptides]

Hi all, I have a question which I'm currently quite confused on.

 

To begin, a saturation binding assay on intact wild type AtnR cells using the tritiated AtnR angatonist [³H]DPPX has been performed. [³H]DPPX is added to cells in increasing concentrations in the presence or absence of cold competitor DPPX to determine the non-specific binding (NS) and total binding (TB), respectively. Each tube contains 1⁵ cells in a final volume of 500µL. DPM (disintegrations per minute) results are obtained for each concentration.

 

From this, I must calculate the specific binding for each concentration.

 

To calculate the specific binding I'll first have to calculate the non-specific binding, and then minus this from the calculated total binding. However, I don't understand how to obtain these from the DPM results?

 

Any help on this would be much appreciated.