GCMS Analysis of Rapamycin

[MDCLS]

Hi All

I am currently trying to analyse Rapramycin (Sirolimus).I have a DES and I am trying to extract the Raprmycin with DMSO and analyse it on the GCMS underivatised does anyone have any experience of this analysis

Thanks

 

[John Cuthber]

I wouldn't even try to get that molecule down a gc column. Too big, too polar.

[hypervalent_iodine]

What John Cuthber said. You are not going to get that down a GC column, so please don't go and destroy one by trying. Have you thought of using LC-MS?

[CharonY]

Agreed, GCMS is no good. Depending on what you try to do either simple LC-UV/vis or LCMS would be much better alternatives. Rapamycin is going to contaminate the GC column badly.