Gene expression database? 5-HT transporter protein

[CiMG]

Hello!

 

I am currently dealing with monoamine transporters (in this case serotonin transporter SERT) and their expression at the synaptic cell membrane. More specifically, I am wondering if there is more explicit data on the expression of SLC6A4 gene for the SERT. I would like to understand how long it takes for a SERT protein to be polymerized and how long it remains in the membrane before it is degraded. However, I haven't even found the right place (database? publications?..) to search for this problem.

 

I am approaching this topic with a rather clinical background, so any hint from a biochemist would help me a lot.

 

Thank you! C

[CharonY]

That is not my specialty, but there is unlikely to be a database of protein turnover. To make sense this would generally be in vivo studies that are quite tricky to conduct with a high amount of biological variance, which makes it a bad measure for databases. You may be lucky that someone has measured it for a specific system and you could use that as a rough estimate for yours, and if you are really lucky it may be even the same.

That being said, there are papers such as Vicentic et al Brain Res. 1999 Sep 11;841(1-2):1-10. that provide some (indirect) ideas about turnover rates.

With respect for gene expression to protein production, that is probably not a limiting factor as in most cellular systems the rates are very high. I.e. only few minutes are required from gene induction to the ability to measure the first proteins.