some questions on bacterial conjugation

[linneaha]

Hi, I need answers to some random questions on an experiment about triparental conjugation - it's hard to find explanations which don't require university education.

 

I'd like to know how the replication of the plasmid works when it is transferred (i know about the rolling-circle-replication and the basic principle, but i couldn't find much about the function of the nic/bom-site and the mobilisation protein)

I also need a little more information about the transposition of genes (again, I know the basics)

How can Pseudomonas aeruginosa survive without sugar (meaning, how exactly does the metabolising of amino acids work? which ones can it metabolise?)

If a fertility plasmid has initiated conjugation and has been transferred, can the mobilisable plasmid just be transferred into the recipient afterwards or is there anything else it has to do?

 

I would be extremely grateful if you could take the time to explain this to me, thanks in advance