blazinfury Posted March 29, 2014 Posted March 29, 2014 I was recently reading about the mechanism of gene targeting using single-stranded oligonucleotides. I am familiar with the targeting using TALENs, ZFNs, CRISPR, etc, but my issue is with understanding the mechanism. With the latter targeting technologies, they bind to a particular locus, induce a break and then with a donor plasmid, one can promote repair. However, how does a single stranded oligo induce targeting? Is a particular enzyme or something to that effect added when transfection occurs?
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