Dilution

[jmcnell]

I've been up all day and am kind of frustrated I can't figure this out. I don't want to mess it up before lab and ruin my entire protocol.

 

I need to make a 2mg/100µl solution with 1000mg NaCl, thus I need to deduce the volume needed to mix the NaCl with to

 

give this ratio of 2mg/100µl

 

I thought the formula was (m1)(v1)=(m2)(v2) but that doesn't seem right when I did the calculation.

 

can some please point me into the right direction for this calculation.

 

 

I've been up all day doing exams and I am just whiped out right now..

[studiot]

Well 1000mg is 500 times as much as 2mg

 

So you will need 500 times as much volume as for 2mg ie 500 x 100 microlitres.

[jmcnell]

50 mL or 50,000 microliters correct?

 

Thanks!!