PLASMID DNA ISOLATION

[goldy]

i would like to know whether repeated freeze thawing of plasmid dna affect its intrgrity

 

[CharonY]

Short answer is: yes, most of the time. Depends on a lot of factors, though (e.g. length, purity buffer etc.).

[iRNAblogger]

Are you concerned with the typology of the plasmid? (Such as amount of supercoiled vs relaxed vs nicked?) Because some studies have suggested that temperature changes can affect the supercoiled state of plasmids: http://www.pnas.org/content/81/13/4046.full.pdf+html

and I'm sure that a similar phenomenon could be observed due to freeze-thaw cycles.