Horseradish peroxidase conjugation

[Astindoram]

Hi every one, I have made a conjugation between a IgG and HRP with the sodium periodate method. I have been asked to quantify how much HRP molecules are bound per IgG molecule. I have no idea how to solve the problem.

PLEASE HELP! I thank a lot!

[CharonY]

There are many ways to approach this. But the simplest is to make calibration curve of the peroxidase (how can you do that simply?) and then transfer that approach to an experiment where you add the immunoassay.