Twotails Posted October 4, 2014 Posted October 4, 2014 Hi all, I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer were 'It's just a matter of logic, consider that fragments may overlap'. The question is as follows:Genomic DNA was digested and run on a gel. The gel was blotted and used for Southern hybridisation. The probe was a 2.4 kb XhoI fragment. Using the southern data, determine the restriction map for genomic DNA.Data:Enzyme Approximate Band SizesE ------------- 6.0 ----- 1.8X ------------- 2.4B ------------ >10 ----- 3.6XE ------------ 1.7 ----- 0.7XB ------------ 2.3 ----- 0.1BE ------------ 2.0 ----- 1.6 ----- 0.2Any help with this would be greatly appreciated.Thank you.
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