Twotails Posted October 4, 2014 Share Posted October 4, 2014 Hi all, I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer were 'It's just a matter of logic, consider that fragments may overlap'. The question is as follows:Genomic DNA was digested and run on a gel. The gel was blotted and used for Southern hybridisation. The probe was a 2.4 kb XhoI fragment. Using the southern data, determine the restriction map for genomic DNA.Data:Enzyme Approximate Band SizesE ------------- 6.0 ----- 1.8X ------------- 2.4B ------------ >10 ----- 3.6XE ------------ 1.7 ----- 0.7XB ------------ 2.3 ----- 0.1BE ------------ 2.0 ----- 1.6 ----- 0.2Any help with this would be greatly appreciated.Thank you. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now