Crappy cultures

[rakuenso]

Here are the ingredients i'm using to grow foot fungus and foot bacteria (yes the nasty smelly stuff in your shoes)

 

250ml of Water

25g of Agar Powder

5g of Dextrose

5g of General Store Sugar

5g of Lactose

 

For some reason they all take long times to grow and is almost translucent...

 

fyi i'm a newb to the growing cultures

[YT2095]

you`re lacking a good many other nutrients in that mix, next time, try adding a Chicken stock cube to the mixture. it`s only simple, but it works great

[rakuenso]

Where can I go about obtaining one?

[YT2095]

Where can I go about obtaining one?

 

by "one" I assume you mean a stock cube or boulion?

 

well most supermarkets will sell them, they`re often added to things like soup or rice or stews etc...

 

basicly most good stock cubes or even the stock as a liquid will do just fine

 

just make sure to dissolve it well, filter out and solid impurities and boil well for a good 5 mins.

you`ll not go far wrong

[ed84c]

so what does agar contain if not nutrients?

 

I tried growing some mouth bacteria on agar, nout happened. Nor did a cold virus grow in a test tube on bacon which i attempted.

 

btw nice devil-like post count YT2095

[MulderMan]

agar is just a jelling medium that nutrients are contained in, and bacteria can grow on.

[rakuenso]

So agar powder alone has enough nutrients? if so, why does nutrient agar also exist?

[ecoli]

because different organisms have different nutrient requirements.

[rakuenso]

well considering that its shoe fungus/bacteria that i'm growing, the only appropriate nutrient I can really think of is sweat... and i'm not sure what thats made of.

[YT2095]

primarily salts and water.

 

and a plethora of bacteria and amines

[ecoli]

-some urea too

[rakuenso]

... by obtaining urea, would it suffice i just... you know.....

[MulderMan]

im not sure if they actually feed on the urea or not, it could just be there as a product of sweat. its a good guess that they would feed on the salts but it always helps to have general nutrients in with cultures.

[PiLoT]

also try incubation at 37C. youd be amazed at what a difference that could make the foot environment might be also slightly acidic

[rakuenso]

thx for the replies, i'm going to make an agar composed of juice, milk, slight HCl, table salts, and some urine (eew)

[ecoli]

good luck