rakuenso Posted March 14, 2005 Share Posted March 14, 2005 Here are the ingredients i'm using to grow foot fungus and foot bacteria (yes the nasty smelly stuff in your shoes) 250ml of Water 25g of Agar Powder 5g of Dextrose 5g of General Store Sugar 5g of Lactose For some reason they all take long times to grow and is almost translucent... fyi i'm a newb to the growing cultures Link to comment Share on other sites More sharing options...
YT2095 Posted March 14, 2005 Share Posted March 14, 2005 you`re lacking a good many other nutrients in that mix, next time, try adding a Chicken stock cube to the mixture. it`s only simple, but it works great Link to comment Share on other sites More sharing options...
rakuenso Posted March 14, 2005 Author Share Posted March 14, 2005 Where can I go about obtaining one? Link to comment Share on other sites More sharing options...
YT2095 Posted March 14, 2005 Share Posted March 14, 2005 Where can I go about obtaining one? by "one" I assume you mean a stock cube or boulion? well most supermarkets will sell them, they`re often added to things like soup or rice or stews etc... basicly most good stock cubes or even the stock as a liquid will do just fine just make sure to dissolve it well, filter out and solid impurities and boil well for a good 5 mins. you`ll not go far wrong Link to comment Share on other sites More sharing options...
ed84c Posted March 14, 2005 Share Posted March 14, 2005 so what does agar contain if not nutrients? I tried growing some mouth bacteria on agar, nout happened. Nor did a cold virus grow in a test tube on bacon which i attempted. btw nice devil-like post count YT2095 Link to comment Share on other sites More sharing options...
MulderMan Posted March 14, 2005 Share Posted March 14, 2005 agar is just a jelling medium that nutrients are contained in, and bacteria can grow on. Link to comment Share on other sites More sharing options...
rakuenso Posted March 14, 2005 Author Share Posted March 14, 2005 So agar powder alone has enough nutrients? if so, why does nutrient agar also exist? Link to comment Share on other sites More sharing options...
ecoli Posted March 15, 2005 Share Posted March 15, 2005 because different organisms have different nutrient requirements. Link to comment Share on other sites More sharing options...
rakuenso Posted March 15, 2005 Author Share Posted March 15, 2005 well considering that its shoe fungus/bacteria that i'm growing, the only appropriate nutrient I can really think of is sweat... and i'm not sure what thats made of. Link to comment Share on other sites More sharing options...
YT2095 Posted March 15, 2005 Share Posted March 15, 2005 primarily salts and water. and a plethora of bacteria and amines Link to comment Share on other sites More sharing options...
ecoli Posted March 16, 2005 Share Posted March 16, 2005 -some urea too Link to comment Share on other sites More sharing options...
rakuenso Posted March 18, 2005 Author Share Posted March 18, 2005 ... by obtaining urea, would it suffice i just... you know..... Link to comment Share on other sites More sharing options...
MulderMan Posted March 18, 2005 Share Posted March 18, 2005 im not sure if they actually feed on the urea or not, it could just be there as a product of sweat. its a good guess that they would feed on the salts but it always helps to have general nutrients in with cultures. Link to comment Share on other sites More sharing options...
PiLoT Posted March 19, 2005 Share Posted March 19, 2005 also try incubation at 37C. youd be amazed at what a difference that could make the foot environment might be also slightly acidic Link to comment Share on other sites More sharing options...
rakuenso Posted March 23, 2005 Author Share Posted March 23, 2005 thx for the replies, i'm going to make an agar composed of juice, milk, slight HCl, table salts, and some urine (eew) Link to comment Share on other sites More sharing options...
ecoli Posted March 23, 2005 Share Posted March 23, 2005 good luck Link to comment Share on other sites More sharing options...
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