Paramecium8 Posted October 21, 2014 Share Posted October 21, 2014 I found some microbes that apparently are some type of bacteria and now i cant find protozoans What are those things? Someone know the specie? Why the Protozoans are dying? those things appeared in the water example and later all the protozoans died Link to comment Share on other sites More sharing options...
CharonY Posted October 22, 2014 Share Posted October 22, 2014 I do not see anything recognizable in that image. What magnification is it? Where do you get your samples and is it possible that the water quality is degrading? 2 Link to comment Share on other sites More sharing options...
Moontanman Posted October 22, 2014 Share Posted October 22, 2014 I have to say that at some point i have seen an image similar to that under the microscope in relation to paramecium but my interest in protozoa is limited to growing them as food for other animals and not technically accurate in any way... Link to comment Share on other sites More sharing options...
Paramecium8 Posted October 22, 2014 Author Share Posted October 22, 2014 I do not see anything recognizable in that image. What magnification is it? Where do you get your samples and is it possible that the water quality is degrading? normal river water with lots of natural food (lettuce) and the magnification is 400X in the photo, and i can see in the 800X magnification this things are circles (bacteria) Link to comment Share on other sites More sharing options...
CharonY Posted October 22, 2014 Share Posted October 22, 2014 So you have a bowl or something similar with lettuce in it? If the water just sits there for a longer time, the water quality will change. It is unlikely that bacteria are directly killing your eukaryotes, they are present all the time. However, they actively degrade the organic material in your water sample, resulting in unfavorable growth conditions, if the water is not replenished. 3 Link to comment Share on other sites More sharing options...
Robittybob1 Posted October 22, 2014 Share Posted October 22, 2014 normal river water with lots of natural food (lettuce) and the magnification is 400X in the photo, and i can see in the 800X magnification this things are circles (bacteria) How did you know you were dealing with "protozoa" in the first place? Link to comment Share on other sites More sharing options...
Paramecium8 Posted October 22, 2014 Author Share Posted October 22, 2014 How did you know you were dealing with "protozoa" in the first place? i saw lots of paramecium in the slides and they disappeared and the bacterias replaced they (a lot of bacterias that looks like the ones in the photo) Link to comment Share on other sites More sharing options...
Robittybob1 Posted October 22, 2014 Share Posted October 22, 2014 i saw lots of paramecium in the slides and they disappeared and the bacterias replaced they (a lot of bacterias that looks like the ones in the photo) I know nothing about paramecium but this YT was very odd. Link to comment Share on other sites More sharing options...
Paramecium8 Posted October 22, 2014 Author Share Posted October 22, 2014 So you have a bowl or something similar with lettuce in it? If the water just sits there for a longer time, the water quality will change. It is unlikely that bacteria are directly killing your eukaryotes, they are present all the time. However, they actively degrade the organic material in your water sample, resulting in unfavorable growth conditions, if the water is not replenished. no i used a big bottle and it not stayed for long time just one month (for the last tests to replenish i waited 2 months or more with less water) but possibly the unfavorable conditions killed the eukaryotes but the lettuce is not totally degraded Link to comment Share on other sites More sharing options...
CharonY Posted October 22, 2014 Share Posted October 22, 2014 (edited) The water would change much faster than for the lettuce to literally degrade. Biological activity are ongoing and one month or so is plenty to change water quality (just ask someone with an aquarium how it is going to be without filtration). Obviously this is speculation, as it is not clear how exhaustive your search was. Edited October 22, 2014 by CharonY 1 Link to comment Share on other sites More sharing options...
Moontanman Posted October 23, 2014 Share Posted October 23, 2014 I set up a 75 gallon aquarium two weeks ago with extensive filtration and lighting and I can already see a shift in life forms, a bottle with no aeration for a month... Link to comment Share on other sites More sharing options...
Paramecium8 Posted October 23, 2014 Author Share Posted October 23, 2014 in the first week i only saw Paramecium aurelia and didinium in the second week i saw a lot of species of parameciums and didinium in the third week they started to disappear and now i can only see bacterias, i know now the water dont have growing conditions but anyone known the specie of this bacteria??? Link to comment Share on other sites More sharing options...
Paramecium8 Posted October 23, 2014 Author Share Posted October 23, 2014 (edited) Now I saw the water a lot of times again and the water is not totally deteriorated??? I can see parameciums in the bottom of the bottle but I cant see in the top of the bottle(where the bacteria is located). Edited October 23, 2014 by Paramecium8 Link to comment Share on other sites More sharing options...
CharonY Posted October 24, 2014 Share Posted October 24, 2014 In that case you have just not been searching enough. Also note that Paramecium feeds on bacteria. Link to comment Share on other sites More sharing options...
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