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New antibiotic found - need help determining mode of action


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Antibiotic inhibits flagella movemet and iron uptake (by same pathway) in a bacteria species. Bacteria is grown with NaNO3 and no aeration and this restores bacteria's motility and iron uptake ability (Sidereophore). Also, a high dosage of antibiotic is bateriostatic.

 

So how does this antibiotic function? Also what's the significance of the NaNO3 and the dosage?

 

So far, I've figured out the antibiotic probably functions by inhibiting PMF. But I don't know how exactly it can do that. I say PMF is the key because both flagelle motility and iron uptake systems need it to work.

 

Any help will be much appreciated. Need answers/possible solutions/ideas by Sunday night. Thanks in advance.

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