Melt curve analysis

[newbie17425]

Hello,

 

I want to understand how do i analyze melt curve after my qPCR? What are the different curves?

I am just aware that melt curve analysis is done to check for secondary products or primer dimer formation.

 

I would appreciate your help in understanding the melt curve.

 

Thanks

[CharonY]

What curves the systems gives you is dependent on the system. Typically it is a simple fluorescence (usually normalized) over temp plot with a curve per sample. However, I wonder, if you are supposed to do this experiments is the lab/course not explaining you how the systems work?

[newbie17425]

For some reason i cannot upload a picture here.

 

I just wanted to know what does the peak represents? And if i had secondary products/contamination/primer dimers then i would have extra peaks right?

[StringJunky]

For some reason i cannot upload a picture here.

Click "More Reply Options". bottom right of message input box. Perform in order as shown:

 

Edited June 6, 2015 by StringJunky

[newbie17425]

Thanks for guiding me on how to upload the pictures.

 

Here are the pictures

 

The first picture is of the NTC and the other two are of the 2 unknown samples in triplicates.