Jump to content

Recommended Posts

Posted

Hi,

 

So i have made some serial dilutions and counted my colonies from two plates (replicate plate also) taken the average and that is 30. These plates were from my 10^2 dilution. Overall i had made 7 dilutions from 10^8 to 10^1, and total dilution is 1 in 10. The vol i plated on to the agar plates was 1ml.

So to get CFU per ml in in the original stock solution (10^8), I need to use this formula which is Colony count per plate x dilution factor /vol plated. However, when it says dilution factor do they mean the total dilution factor which is 1 in 10? or the dilution of the actual plate which is 10^2 ?

 

Can someone please just clarify this, I have looked endlessly on the internet and no where does it say

 

Thank you

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.