Determining MIC

[Gamewizard]

Hi all

I basically need some help with interpreting my MIC results.

 

I have carried out a MIC test on some raw materials against my bug. Bacterial inoculum was at 0.6 OD. Used a 96 well A to H microtire plate.

 

Obviously made serial dilutions of the raw materials, now some of my raw materials were liquid so i used them straight away (100%) but some were solids so i had to dilute them first with with sterile water.

 

I made a 1 in 2 dilution so put in 10g of solid raw material in to 20 g water. So thier starting percentage was 50%, and then the serial dilutions were made from Column 2 (total vol of column 2 was 200ul as i had 100ul sterile water and 100ul of raw material in it) by taking 100ul out and transfering to next one and so on. (1 in 2 dilution so the percentage was halved each time like so):-

 

Raw material A

100%, 50% 25% 12.5%, 6.25%, 3.125%, 1.5625%, 0.78174%, 0.390625%, 0.1953125%, 0.09765625%, and 0.0244140625% for all wells.

 

Now i need to determine at what concentrations the raw materials inhibited the bacterial growth and I dont know how to figure out the concentration, and i believe the concentration has to be in ug/ml as well ?

 

Can some one please help me figure this out, I have looked on the internet but it has confused me and I do not understand it.

 

Thank you for reading