Optical density = concentration

[Gamewizard]

Hi quick question

 

What would be the bacterial concentration at 0.6 OD ? and how would you write that as ?

 

thank you

[Enthalpy]

Hi, OD=0.6 only means that the power of light passing through is divided by 10^0.6. You would need the light's path length and some attenuation (possibly by diffusion) properties of these specific bacteria to deduce a concentration, provided nothing else attenuates.

[CharonY]

That is absolutely dependent on your bacterium and the medium you are using. You will either have to count the cells or plate them (life titer) for a given OD range and plot a calibration curve.

[Gamewizard]

Thank you for your replies, I will have to do plate counts for it