Western Blot for p-STAT1 and p-STAT6

[Tran Phuong Thao]

This is the first time I do WB with p-STAT1 or p-STAT6 for inflammation.

p-STAT1 (tyr701) and p-STAT6(Tyr641) and both are from Santa Cluz Biotechnology.

 

Treatment of LPS+IFN-y for expression of p-STAT1 , and IL-4 for p-STAT6.

I try to WB many time but it still has no band.

Is there anyone having this experiment can give me the conditions for WB these protein?

Thank you very much!