Silvia_84 Posted January 19, 2016 Posted January 19, 2016 Hi all, I am encountering difficulties trying to figure out some calculations I have to do in order to diluite (or concentrate) my 2 bacterial solutions for a MIC test. I should adjust the density of my overnight cultures with water to equal that of the 0.5 McFarland Standard, which is roughly equivalent to OD600 0.08-0.1 (107-108 cfu/ml). I diluted my original overnight cultures like this: 1900 ul of water and 100 ul of overnight culture (total: 2 ml) and I take 1 ml of this solution for the measurements at the spectrophotometer. One culture has an OD of 1.10, so I have to diluite it and the other 0.06 so I should concentrate it. My question is : how do I diluite the first ? and viceversa..for the second..how do I concentrate it? which calculation should I do? Any suggestion would be really appreciated. Thank you in advance. Silvia
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now