digital PCR & measure stage

[Nubellybus]

Hi all,

 

I've a query about what stage fluorescence is measured at in a digital PCR reaction.

Is it continuous or only at the end of the reaction?

 

i thought the former, but I'm after reading a paper stating the latter in a matter of fact way.

 

Can it be both??

 

Thanks guys!!!

[NucleotidalWave22]

It measures before, during and after in order to quantify the rate in which the PCR has multiplied the sample.

[CharonY]

Digital PCR pretty much only refers to the partitioning of the template prior to the reaction. As it can have many different applications, there can be differences when measurements occur. Even in cases of qPCR it is not necessary to monitor continuously as in standard approaches. Rather, the amount of negative to positive partitions and Poisson statistics can be used to calculate amount. In this case an endpoint measurement would suffice.