Silvia_84 Posted February 29, 2016 Posted February 29, 2016 Hi all, I am new with Microbiology laboratory stuff and I am doing some MICs with microbroth dilution method. I have a problem with that because I obtain MIC values for my control strains higher than expected (2-3 fold higher) and I can't understand what does not work in my experiment. I thought that it could be a diminished antibiotic potency but the drug is not out of date. I thought that it could depend on the solvent I use to make the antibiotic dilutions..I am doing them in Iso Sensitest broth. The procedure I used so far for my MIC is always the same and it worked with antibiotics like Ampicillin and Meropenem. Now I changed antibiotic and I am using Amikacin and Gentamicin and for both the drugs I always have MIC higher than expected. Could you suggest me what might be the problem? Thank you very much in advance. Best, Silvia
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