Silvia_84 Posted February 29, 2016 Share Posted February 29, 2016 Hi all, I am new with Microbiology laboratory stuff and I am doing some MICs with microbroth dilution method. I have a problem with that because I obtain MIC values for my control strains higher than expected (2-3 fold higher) and I can't understand what does not work in my experiment. I thought that it could be a diminished antibiotic potency but the drug is not out of date. I thought that it could depend on the solvent I use to make the antibiotic dilutions..I am doing them in Iso Sensitest broth. The procedure I used so far for my MIC is always the same and it worked with antibiotics like Ampicillin and Meropenem. Now I changed antibiotic and I am using Amikacin and Gentamicin and for both the drugs I always have MIC higher than expected. Could you suggest me what might be the problem? Thank you very much in advance. Best, Silvia Link to comment Share on other sites More sharing options...
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