calculations and deuterium

[aj25]

For deuterated NAD²H is the first metabolite of gluconeogenesis to incorporate the label G3P?

 

1. Asked to calculate the moles of protons pumped out of the mitochondrial matrix per mol of FMN molecule as electrons are passed on to coenzyme-Q. First I used this equation:

∆G = -2.303RT(pH_out – pH_in) + ZF∆yto calculate kj/ mol of protons and that's where I got stuck and don't know what to do next and ∆Gº’= -73.3 kJ/mol ∆pH= 1.3 ∆y= 0.18V and T= 37C. Please help!

 

2. Asked to calculate how many molecules of FMNH₂ need to get oxidized to make 450 g ATP. DG for hydrolysis of ATP is -50.2 kJ/mol and the efficiency of ATP synthase is 40.2% and MM of ATP= 503.15g/mol.

 

For this question i began by calculating the moles of ATP and than I multiplied the DG for hydrolysis of ATP with the efficiency and thats as far as I got. I'm not sure what to do next.

[BabcockHall]

By G3P, what do you mean? What are your thoughts with respect to this question?

 

For your other questions, I think you will have to look into the stoichiometry of how many electrons it takes per ATP synthesized.

[aj25]

by G3p I mean Glyceraldehyde 3-phosphate and i thought it would be that because i assumed NADH was that same as NAD²H and G3P is the only metabolite of gluconeogenesis that uses it. But i wasn't sure about the answer because the question continues to ask why the deuterium label taken up by G3P may not show up in the final product of glucose which i don't really understand.

[BabcockHall]

I would ask whether any subsequent reaction might remove the deuterium label. You will probably have to examine the chemical mechanisms by which these enzymes work, starting with triosephosphate isomerase and aldolase.

Edited March 8, 2016 by BabcockHall