GnothiSeauton Posted March 6, 2016 Posted March 6, 2016 Hi all I have a question concerning transcription Problem goes like this: If RNA pol transcribes DNA from right to left, write newly sinthetised mRNA along with orientations. This is DNA: 5'-ATATCCCTAATCCG-3' 3'-TATAGGGATTAGGC-5' I understand that RNA pol can't go from left to right in direction 3'-5', thus we are talking about transcribing DNA which goes in 5-3 direction. So this is what I got a mRNA, and final solution to this problem: 3'-UAU AGG GAU UAG GC 5' Is this correct?
Xalatan Posted March 7, 2016 Posted March 7, 2016 Unfortunately no. 5'-->3' is the coding strand, while 3'-->5' is the template strand. RNA pol binds to the template strand, and transcribes in the complementary 5'-->3' direction, so the resultant mRNA reads 5'-->3', the same as the coding strand (which is why the coding strand is so named). The mRNA should read the same as the coding strand, except U subbed for T.
BabcockHall Posted March 11, 2016 Posted March 11, 2016 I read this question differently from Xalatan. IMO if the polymerase moves right to left, it will treat the top strand as the template strand.
Xalatan Posted March 11, 2016 Posted March 11, 2016 https://en.m.wikipedia.org/wiki/Coding_strand https://www.google.com.tw/search?q=coding+strand&oq=coding+strand&aqs=chrome..69i57j0l3j69i60.4917j0j4&client=ms-android-htc-rev&sourceid=chrome-mobile&ie=UTF-8#imgrc=N_H8uoPZ_vi3aM%3A
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