Silvia_84 Posted March 7, 2016 Share Posted March 7, 2016 Hi all,can you tell me how I prepare a stock solution of 10% SDS? What does 10% mean in this case? 10mg in 100 ml?10 g in 100 ml ?Thank you very much.Silvia Link to comment Share on other sites More sharing options...
CharonY Posted March 7, 2016 Share Posted March 7, 2016 Do you have protocol? If so concentrations should always be indicated by weight and/or volume (e.g. w/w, v/w, v/v). Of course in case of solids weight is usually used. Link to comment Share on other sites More sharing options...
Silvia_84 Posted March 8, 2016 Author Share Posted March 8, 2016 I have a protocol but it says just to add 40 ul of 10% SDS . The SDS I have is in powder.. So, I can suppose that I have to weight 10 g and dissolve them in 100 ml, haven't I? Link to comment Share on other sites More sharing options...
foxy john Posted March 30, 2016 Share Posted March 30, 2016 SDS is a solid, so a 10% solution will be 10% weight of SDS in water, 10g per 100ml The weights and volumes aren't critical. Are you a student or working in a lab? What background do you have? Link to comment Share on other sites More sharing options...
CharonY Posted March 30, 2016 Share Posted March 30, 2016 SDS is a solid, so a 10% solution will be 10% weight of SDS in water, 10g per 100ml The weights and volumes aren't critical. Are you a student or working in a lab? What background do you have? What do you mean by the weight and volumes are not critical? Or do you mean that the difference for water between weight and volume percentages is not critical? Link to comment Share on other sites More sharing options...
foxy john Posted April 1, 2016 Share Posted April 1, 2016 Just that 10% SDS need not be made up volumetrically. You are not going to get much difference in action between 9% and 11%, I used to make it up 10g +-0.2g in 100ml water. Link to comment Share on other sites More sharing options...
CharonY Posted April 1, 2016 Share Posted April 1, 2016 That depends a lot on your application. I doubt that it is the case for OP but if you need quantitative reproducibility or are investigating high-resolution interactions with surfactants you better be more precise. If you want to work at the critical micelle concentration, for example being even 1mmol off can be problematic, depending on your analysis. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now