Agarose gel for a second run

[Silvia_84]

Hi all,

have you ever tried to re-run an agarose gel after the run is finished and after visualizing it under UV? Last time I run a gel for a hour but the bands were not separate properly yet. I made the gel again but maybe (for a next time)it is possible to put the same gel on a second run after the analysis? Or does it get degraded?If yes, can you also explain me why?

Thank you very much.

Silvia

[Xalatan]

Yeah it's fine, the background will be stronger with the EtBr on the second run but if you just want to check for a band it is still useable. Maybe drop the exposure time a bit and see if it's more optimised.

Let the gel cool down a bit though, you don't want the gel to melt if it overheats.