bacterial freezer stock with beads

[Silvia_84]

Hi guys,

 

I have two questions about the preparation of bacterial freezer stock with beads.It is new for me. How can I make it?Can you indicate me the procedure/ steps to carry it out?

When I have to streak the bacteria from the frozen beads , how is it better to do it? roll the beads on an agar plate or just use a loop to touch the beads and pass it on the plate?

 

Thank you very much.

 

 

[HootersGeek]

This is one of the best places to order beads: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CryoBeads.htm

 

You will need these special porous beads if you want successful long term storage of stock cultures.

 

When you go to streak it onto a plate, you should roll 1 bead on a nonselective agar (blood, chocolate, tsa) in the first quadrant (with a sterile loop or something), sterilize or change loops, then use the loop to do a 4 quadrant streak for isolation (to make sure it's not contaminated) and incubate 18 hours. If you're needing it for susceptibilities, the first pass (initial streak plate from beads) is not optimal to use right off. Take a colony from the first sub plate and sub again to a non-selective agar incubate 18 hours. This 2nd plate is your working organism.

 

Hope this wasn't too late!