Silvia_84 Posted April 14, 2016 Posted April 14, 2016 Hi all, I tried to do multiplex PCR to amplify more genes at once with the biomix red polymerase. Unfortunately it did not work (no bands at all, not even primer dimers)and I have no idea of what goes wrong. The primers should be ok because I am using those already used by another group who has already done the same multiplex PCR.I don't ' know if I make some mistake in the procedure: For a 25 ul of reaction mixture I added: 12.5 ul of BIOMIX RED 1 ul of each primer (they are in total 8 because the genes I am amplifying 4 genes together) 3.5 ul of water The single PCR, done with the primers separately is fine.. so there must be something wrong in the multiplex set-up Thank you in advance.
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