Pericentromere specific probes?

[Matan Yadgar]

Hi all,

Im trying to do a FISH on DNA combing for pericentromeric regions.

As you may know, the human centromere is actually separated into two distinct regions: 1) centromeric region - which is involved in kinetochore binding 2) pericentromere region - a heterochromatic region which is involved in sister chromatid binding.

Im looking for pericentromere specific probes which do not overlap with any other region (centromeric or non centromeric).

Does anyone have a clue whether this kind of probes/BAC/HAC/anything else exist? if so - a link would be great!

Thank you!

Matan

Edited May 18, 2016 by Matan Yadgar