how to check the effects of a change in the nucleotidic sequence

[Silvia_84]

Hi all,

 

I got nucleotide sequences of a PCR product corresponding to a specific variant of an enzyme I am studying. I made a search of homologies by BLAST to see if the gene I have shows 100% homologies with already known variants or if it is a new variant. I found some changes in the sequence and I would like to know if this change is just a silent mutation or it leads to a different amino acid sequence. How can I check this? Do you know good softwares for the translation of the DNA sequence in amino-acids?

 

Thanks

[hypervalent_iodine]

I would think that converting to amino acid sequence is fairly trivial if you already have the reading frame. There should be plenty of programs online that do this. http://web.expasy.org/translate/ For example.