Fused protein properties prediction

[Ploab]

Hi! I have a problem with a fused protein. I want to know the optimal pH and temperature range (among other physical properties) of a fused protein, made of two mini-proteins. However, I'm still waiting for my DNA synthesis to arrive in order to produce it and I only have a few properties of the mini-proteins.

 

I do have my fused protein modelling and structure, but I cannot predict any of these parameters. Does anyone know about how can I predict (in the most accurate possible way) pH, temperature and isoelectric point of my fused protein without experimenting? Thank you!

[BabcockHall]

It is possible to estimate the pI of any protein of known sequence. Just import the sequence into a pI calculation routine, such as the one at ExPASy.

[Justin.Frank]

You can use some software to predicate the basic information of your protein.

I think the following site may be useful for you：

https://en.wikipedia.org/wiki/List_of_protein_structure_prediction_software

 

Or you can seek help from some commercial company such as Predictprotein and Creative Biostructure.