Can I predict if a gene sequence will be difficult/have low yield in culture?

[alex.mil]

Hello all! I am trying to work out if I can predict if a sequence is going to be difficult to grow in a culture. I know that things such as size (like in the muscular dystrophy gene) and secondary structures (like ITRs in AAV) famously make gene sequences very hard to yield in culture. Are there a number of elements I should look for that will help me predict if something will be difficult? Are there other examples of very difficult genes you can think of? Maybe G-protein coupled receptors? Do we know why these are so difficult, and what they do to the cells during culture?

Thanks!