hyebeh Posted May 1, 2005 Posted May 1, 2005 Why do you need a phosphate buffer when you run chloroplasts through a spectrophotometer to test for photosynthesis? Is it because the phosphate allows the chloroplasts to form ATP from ADP (in other words, providing the reducing agent)?
hyebeh Posted May 3, 2005 Author Posted May 3, 2005 i think i answered my own question. I believe that the phosphate buffer is used to maintain a constant pH (that's what buffers are for! lol)
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